Tetracarpidium conoforum, (family-Euphorbiaceae), root bark was investigated for its Phytoconstituents and chemical characterization by high performance liquid chromatography (HPLC). The root bark was extracted by hexane and concentrated for further analysis. A portion of the crude extracts recovered by rotary evaporator was screened for their phyto constituents using standard methods and the other portion isolated by vacuum liquid chromatograpy (VLC) was characterized by infra red spectroscopy and HPLC. Phenolics, terpenoids and flavonoids were present in the hexane extract while lupeol and juglone were detected as major chemical components. This research indicated the presence of phyto-constituents with proven important physiological activities.