Cryptococcus neoformans is a pathogenic fungus that produces melanin pigmentation,which is a major virulence factor that contributes to its pathogenicity. C. neoformans strains with varying levels of melanin production were screened and selected to evaluate their growth and melanin production under different temperature ranges. This was carried out in order to ascertain the optimal conditions for melanin production and understand the effects of temperature on the phenotypic expression of C. neoformans. Out of the 150 isolates that were tested for melanin production, 10 strains of C.