Human activities have immensely contributed to pollutants in water bodies such as heavy metals, organic and pathogenic microorganisms. This study was aimed to synthesize and investigate the aseptic efficacy of ZnO-carbonized zobo waste nanocomposite (ZOCZWNC) and Mg-Zn binary oxide (Mg-ZnBO) nanocomposite on Salmonella typhi – gram negative organism and Staphylococcus aureus – gram positive organism as well as removal of cadmium and nickel ions from contaminated water. Mg-Zn binary oxide (Mg-ZnBO) Nanocomposite and ZnO–carbonized zobo waste nanocomposite (ZnOCZWNC) was prepared and characterized. Adsorption investigation was done using adsorption isotherm and the aseptic efficiency of ZnOCZWNC and MgZnBO nanocomposites was studied using turbidimetric method. Maximum sorption capacity of cadmium onto ZnOCZWNC and Mg-ZnBO was 9.11mg/g and 192.31mg/g respectively and 714.29 mg/g and 416.67 mg/g in that order for nickel ions. The average crystallite size of ZnOCZWNC and MgZnBO was 40.94 nm and59.42nm respectively. The treated samples experienced a steady decrease in the population of Staphylococcus and Salmonella within 2hrs, after about 2hrs, the samples of staphylococcus and salmonella treated with ZnOCZWNC continued to show continuity in its aseptic effect at decreasing the population of the bacteria with time. The staphylococcus and salmonella sample treated with MgZnBO experienced a slightly steady drop in the effectiveness of the nanocomposite after same period. The ZnOCZWNC removed nickel ions more effectively compared to Mg-ZnBO, conversely, MgZnBO removed cadmium ions more effectively compared to ZnOCZWNC. ZnOCZWNC was more efficient in drastically reducing the population of staphylococcus and salmonella in contaminated water.